Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterizat...

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Published inJournal of Veterinary Medical Science p. 21-0234
Main Authors SAHASHI, Yuma, OHSHIMA, Masaaki, YAMAGISHI, Jyunjirou, MURAMATSU, Chieko, SHIMIZU, Kaori, INOSHIMA, Yasuo
Format Journal Article
LanguageEnglish
Published JAPANESE SOCIETY OF VETERINARY SCIENCE 01.11.2021
Subjects
bovine leukemia virus
epidemiological analysis
genotype
polymerase chain reaction-restriction fragment length polymorphism
sequencing
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Summary:Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences and were, therefore, not new genotypes.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.21-0234