A novel platform technology for the detection of genetic variations by surface plasmon resonance

We report on a novel approach to identify genetic variations based on the detection of specific polymerase-chain-reaction products by surface plasmon resonance. We use a recently developed, home-made spectrometer which exploits chips with integrated optics and microfluidics. The gold film at the chi...

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Bibliographic Details
Published in2009 IEEE Sensors pp. 392 - 395
Main Authors Mertig, M., Kick, A., Bonsch, M., Katzschner, B., Voigt, J., Sonntag, F., Schilling, N., Klotzbach, U., Danz, N., Begemann, S., Herr, A., Jung, M.
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.10.2009
Subjects
Genetics
Gold
Integrated optics
Microfluidics
Optical films
Plasmons
Polymers
Probes
Resonance
Spectroscopy
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Summary:We report on a novel approach to identify genetic variations based on the detection of specific polymerase-chain-reaction products by surface plasmon resonance. We use a recently developed, home-made spectrometer which exploits chips with integrated optics and microfluidics. The gold film at the chip surface is locally functionalized with single-stranded, thiol-modified probe DNA by applying a nanoliter dispenser. We discuss the chemical conditions for the achievement of maximum SPR signal strength and spot array density, and evaluate the SPR detection of selective binding of a set of 17 PCR products.
ISBN:1424445485
9781424445486
ISSN:1930-0395
2168-9229
DOI:10.1109/ICSENS.2009.5398247