Silylation Derivatization for the Detection of Hydroxylated Polychlorinated Bipheny in Aquatic Products by GC/MS

The present study established a method to determine 7 hydroxy metabolites of polychlorinated biphenyls (PCBs) in aquatic products.Target compunds were extracted by hexane/ethyl acetate (v/v,1:1). Sulfuric acid and silica column were used to remove the impurities with 50 ml of hexane/ethyl acetate (v...

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Published in2009 3rd International Conference on Bioinformatics and Biomedical Engineering pp. 1 - 4
Main Authors Yong-Fu Shi, Dong-mei Huang, Youqiong Cai, Yuan Wang, Hong Lin
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.06.2009
Subjects
Aquaculture
Biological materials
Calibration
Glass
Impurities
Oceans
Silicon compounds
Solvents
Temperature
Testing
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Summary:The present study established a method to determine 7 hydroxy metabolites of polychlorinated biphenyls (PCBs) in aquatic products.Target compunds were extracted by hexane/ethyl acetate (v/v,1:1). Sulfuric acid and silica column were used to remove the impurities with 50 ml of hexane/ethyl acetate (v/v,1:1) as eluet. Silylation derivatization was effective and stable to make hydroxylated polychlorinated biphenyls more volitile for GC/MS analysis. The coefficients of derivivatives ranged from 0.996 to 0.999 in the range of 1-100 ng/g. Recoveries of spiked tissues prepared with this method ranged from 76.7% to 106%,and the relative standard deviations (RSD) ranged from 4.0% to 11.1%. The method was applied to determine hydroxy-PCBs in aquatic products sampled from local market.
ISBN:9781424429011
1424429013
ISSN:2151-7614
2151-7622
DOI:10.1109/ICBBE.2009.5163385