Establishment of a Bioinformatics Method for Detecting of ESBLs Producing Isolates of Pseudomonas aeruginosa at Different Surgical Wards

• Published in: 2012 International Conference on Biomedical Engineering and Biotechnology pp. 256 - 259
• Main Authors: Zhi-long Cheng, Hui Niu, Guang-yu Cheng, Yan-suo Jiang, Ming-cheng Li
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01.05.2012
• Subjects: Antibiotics; DNA; Expended spectrum-ß-lactamases; Hospitals; Immune system; Microorganisms; nosocomial infection; Pseudomonas aeruginosa; Strain; Surgery
• ISBN: 1457719878; 9781457719875
• DOI: 10.1109/iCBEB.2012.200

This study was carried out to explore the molecular occurrence and dissemination of P. aeruginosa producing ESBLs recovered from the different wards in the affiliated hospital, Jilin. Antibiotic susceptibility test was conducted to use K-B disc diffusion in conjunction with ESBLs screening methods. Plasmid profiles were used to study the relatedness of isolates and bioinformatics method was used to detect of the class A-lactamase genes. Of all isolates, 91 strains were isolated from wounds at burn wards and 149 strains from wounds at surgery wards. The total of 210 strains (87.5%) produced ESBLs, 30 strains (12.5%,) didn't produce ESBLs. All ESBLs isolates showed identical antimicrobial susceptibility profiles. 37 strains (17.6%) were positive for bla SHV-12 gene, 43 strains (20.5%) contained bla TEM-24 gene, and 30 strains (14.3%) had bla CTX-M-1 gene, 20 strains(9.6%) contained bla CTX-M-2 gene, 27 strains(12.9%) contained bla CTX-M-3 gene, 29 strains(13.8%) contained bla PER gene, and 24(11.4%) strains contained blaVEB gene respectively. The data indicated a high prevalance of ESBL among Pseudomonas aeruginosa in this region and their enzyme types were diverse.