An optical platform based on fluorescence anisotropy for C - reactive protein assay

A novel optical platform for point of care testing application was developed. The core of the platform is a miniaturised polymethylmetacrylate (PMMA) chip through which the analysed sample flows. Thanks to the fluorescence anisotropy exhibited by any dipole emitting at a distance from a medium inter...

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Bibliographic Details
Published in2008 IEEE Sensors pp. 1198 - 1201
Main Authors Baldini, F., Carloni, A., Giannetti, A., Trono, C., Porro, G., Tedeschi, L.
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.10.2008
Subjects
Anisotropic magnetoresistance
Diseases
Fluorescence
Geometrical optics
Optical fibers
Optical sensors
Plastics
Proteins
Stimulated emission
Testing
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Summary:A novel optical platform for point of care testing application was developed. The core of the platform is a miniaturised polymethylmetacrylate (PMMA) chip through which the analysed sample flows. Thanks to the fluorescence anisotropy exhibited by any dipole emitting at a distance from a medium interface of the order of the emitted wavelength, a large fraction of the fluorescence emitted by the sensing layer immobilized onto the internal flow-channel travels along the thickness of the PMMA up to its end-face where it is collected by a plastic optical fibre connected to a spectrum analyser. The potentiality of the optical chip was investigated with a sandwich assay for the C-reactive protein, one of the markers for inflammatory diseases.
ISBN:1424425808
9781424425808
ISSN:1930-0395
2168-9229
DOI:10.1109/ICSENS.2008.4716657