Difference proportional cell contact platform for 3D hepatocyte culture

• Published in: 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) pp. 191 - 194
• Main Authors: Yu-Shih Chen, Zong-Keng Kuo, Ling-Yi Ke, Chiou-Wen Lin, Hsiang-Wen Tseng, Cheng-Hsien Liu
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01.03.2012
• Subjects: Barium; cell pattern; Dielectrophoresis; Liver; Loading; Microfluidics Circulatory System; Photonics; Titanium
• ISBN: 1467311227; 9781467311229
• DOI: 10.1109/NEMS.2012.6196754

This paper reports a difference proportional cell contact platform for three dimension hepatocyte culture. We designed the cell wires about the width of 25 micrometer by dielectrophoresis for difference contact area. The platform was consisted of two dimensional cell contact chambers and three dimensional liver tissue chambers by dielectrophoresis pattern method and microfluidic channel design. For cell contact chambers, we used dielectrophoresis pattern method to make difference contact area for difference proportional cell communication. For liver tissue chambers, we used the UV-initiated solution of the poly (ethylene glycol)-diacrylate (PEG-DA) had been exposed to form microwells to pattern three dimension lobule-mimetic structure liver tissue in vitro. HepG2 cells were loading with three dimensional PEG-DA-dased micro-structures for artificial lobule pattern. Finally, we showed liver functional albumin testing. The albumin secretion of liver pattern chambers with HMEC-1 and C2C12 co-culture communication was 34% higher than the albumin secretion of liver chip without cells communication.