Optimal Fed Batch Experiment Design for Estimation of Monod Kinetics of Azospirillumbrasilense: From Theory to Practice

• Published in: Biotechnology progress Vol. 23; no. 5; pp. 1074 - 1081
• Main Authors: Cappuyns, Astrid M., Bernaerts, Kristel, Smets, Ilse Y., Ona, Ositadinma, Prinsen, Els, Vanderleyden, Jos, Van Impe, Jan F.
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 2007
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp070046x

In this paper the problem of reliable and accurate parameter estimation for unstructured models is considered. It is illustrated how a theoretically optimal design can be successfully translated into a practically feasible, robust, and informative experiment. The well‐known parameter estimation problem of Monod kinetic parameters is used as a vehicle to illustrate our approach. As known for a long time, noisy batch measurements do not allow for unique and accurate estimation of the kinetic parameters of the Monod model. Techniques of optimal experiment design are, therefore, exploited to design informative experiments and to improve the parameter estimation accuracy. During the design process, practical feasibility has to be kept in mind. The designed experiments are easy to implement in practice and do not require additional monitoring equipment. Both design and experimental validation of informative fed batch experiments are illustrated with a case study, namely, the growth of the nitrogen‐fixing bacteria Azospirillum brasilense.