CD49f promotes proliferation of male dairy goat germline stem cells
Objectives CD49f enhances multipotency and maintains stemness in embryonic stem cells (ESCs), however, whether it would be effective in mGSCs has remained unclear. Moreover, better standards for mGSC enrichment and purification are necessary. The present study was conducted to determine roles of CD4...
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Published in | Cell proliferation Vol. 49; no. 1; pp. 27 - 35 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.02.2016
John Wiley and Sons Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Objectives
CD49f enhances multipotency and maintains stemness in embryonic stem cells (ESCs), however, whether it would be effective in mGSCs has remained unclear. Moreover, better standards for mGSC enrichment and purification are necessary. The present study was conducted to determine roles of CD49f in mGSC enrichment and regulation.
Materials and methods
CD49f expression patterns were investigated in dairy goats. CD49f positive cells were purified and enriched using magnetic‐activated cell sorting (MACS), and characteristics of the cultured cells were assayed using alkaline phosphatase (AP) analysis, quantitative real‐time PCR (QRT‐PCR) and immunofluorescence analysis. Furthermore, the exogenous CD49f gene was transfected into mGSCs and its effects were analysed.
Results
CD49f was found to be conserved in both mRNA and amino acid sequences and that it was an efficient marker for dairy goat mGSC identification, enrichment and purification. CD49f positive cells expressed higher levels of mGSC‐specific markers, and proliferated faster than CD49f negative cells. Overexpression CD49f promoted proliferation of dairy goat mGSCs, and Oct4 expression was upregulated; histone H3‐lysine 9 dimethylation (H3K9me2) was reduced.
Conclusions
Taken together, our data suggest that CD49f plays novel and dynamic roles in regulating maintenance of pluripotency in mGSCs via Oct4 crosstalk and histone methylation dynamics,which may provide new solutions for mGSCs stability in vitro. |
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Bibliography: | ark:/67375/WNG-KBL2DV9X-4 National Major Project for Production of Transgenic Breeding - No. 2014ZX08007-002 Program of National Natural Science Foundation of China - No. 31272518; No. 31572399 istex:276A2453962D7A89BCA6B1D677C5324EFC6AB62D National High Technology Research and Development Program of China - No. SS2014AA021605 Key Project of Chinese Ministry of Science and Technology - No. 2013CB967401 Table S1 The sequence of dairy goat CD49f. Doctoral Fund of Ministry of Education of China - No. 20120204110030 ArticleID:CPR12232 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors equally contribute to this study. |
ISSN: | 0960-7722 1365-2184 |
DOI: | 10.1111/cpr.12232 |