Analysis of Amblyomma sculptum haplotypes in an area endemic for Brazilian spotted fever
Amblyomma sculptum (Ixodida: Ixodidae) Berlese, 1888, a member of the Amblyomma cajennense complex, is the major vector of Brazilian spotted fever (BSF) in southeastern Brazil. In this study, the genetic diversity of A. sculptum populations in the state of Rio de Janeiro (RJ), Brazil, was investigat...
Saved in:
| Published in | Medical and veterinary entomology Vol. 30; no. 3; pp. 342 - 350 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.09.2016
Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Summary: | Amblyomma sculptum (Ixodida: Ixodidae) Berlese, 1888, a member of the Amblyomma cajennense complex, is the major vector of Brazilian spotted fever (BSF) in southeastern Brazil. In this study, the genetic diversity of A. sculptum populations in the state of Rio de Janeiro (RJ), Brazil, was investigated because genetic variability in tick populations may be related to vector competence. Samples of A. sculptum from 19 municipalities in 7 regions of RJ were subjected to DNA extraction, amplification and sequencing of D‐loop, cytochrome oxidase II and 12S rDNA mitochondrial genes. These sequences were used to map the genetic diversity of this tick. Amblyomma sculptum populations are genetically diverse in RJ, especially in the South Centre and Highland regions. Few unique haplotypes were observed in all populations, and the majority of genetic variation found was among ticks within each population. Phylogenetic reconstruction reinforced the assumption that all the haplotypes identified in RJ belong to A. sculptum. However, some RJ haplotypes are closer to A. sculptum from Argentina than to A. sculptum from elsewhere in Brazil. In RJ, A. sculptum has high genetic diversity, although little genetic differentiation. Observations also indicated a high level of gene flow among the studied populations and no evidence of population structure according to region in RJ. |
|---|---|
| Bibliography: | Conselho Nacional de Desenvolvimento Científico e Tecnológico [CNPq (National Council of Technological and Scientific Development)] - No. Universal 481843/2013-2 Figure S1. Phylogeny inferred by maximum likelihood analysis of mitochondrial 12S rDNA gene, representing the relationships between haplotypes (I-V) of Amblyomma sculptum identified in the state of Rio de Janeiro, Brazil and species of Amblyomma cajennense complex from different locations in the Americas. Numbers on the branches represent support values (70% cut-off).Figure S2. Phylogeny inferred by maximum likelihood analysis of mitochondrial cytochrome oxidase II gene, representing the relationships between haplotypes (1-8) of Amblyomma sculptum identified in the state of Rio de Janeiro, Brazil and species of the Amblyomma cajennense complex from different locations of the Americas. Numbers on the branches represent support values (70% cut-off).Figure S3. Phylogeny inferred by maximum likelihood analysis of mitochondrial D-loop gene, representing the relationships between haplotypes (A-H) of Amblyomma sculptum identified in the state of Rio de Janeiro, Brazil and species of the Amblyomma cajennense complex from different locations in the Americas. Numbers on the branches represent support values (70% cut-off).Table S1. Origin of samples of Amblyomma sculptum collected in different regions in the state of Rio de Janeiro, Brazil, from 2005 to 2011. Fieldwork was conducted in partnership with teams from the Laboratório de Referência Nacional em Vetores das Riquetsioses, FIOCRUZ, Rio de Janeiro, Brazil, and the Secretaries of State and Municipal Health of RJ.Table S2. Composition of sample mixtures and polymerase chain reaction amplification cycles for the analysed genes of Amblyomma sculptum collected from different regions in the state of Rio de Janeiro, Brazil.Table S3. Geographic region, haplotype and associated GenBank accession numbers for Amblyomma sculptum (= Amblyomma cajennense) analysed samples. Total numbers of ticks from each locality and with identical sequences are also shown.Table S4. GenBank accession numbers of the 12S rDNA gene sequences used in the phylogenetic reconstruction of the Amblyomma cajennense complex.Table S5. GenBank accession numbers of the cytochrome oxidase II gene sequences used in the phylogenetic reconstruction of the Amblyomma cajennense complex.Table S6. GenBank accession numbers of the D-loop gene sequences used in the phylogenetic reconstruction of the Amblyomma cajennense complex.Table S7. Distribution and frequency of Amblyomma sculptum D-loop haplotypes from different regions in the state of Rio de Janeiro, Brazil.Table S8. Distribution and frequency of Amblyomma sculptum cytochrome oxidase II haplotypes from different regions in the state of Rio de Janeiro, Brazil.Table S9. Distribution and frequency of Amblyomma sculptum 12S rDNA haplotypes from different regions in the state of Rio de Janeiro, Brazil. istex:C9E531217C668D19754263F04242382C66EAE6B3 ark:/67375/WNG-7CM3FMGH-Z Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro [FAPERJ (Foundation for Support to Research in the State of Rio de Janeiro)] - No. APQ1 E-26/111.719/2012 ArticleID:MVE12174 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0269-283X 1365-2915 |
| DOI: | 10.1111/mve.12174 |