Endomembrane Ca2+-ATPases play a significant role in virus-induced adaptation to oxidative stress

• Published in: Plant signaling & behavior Vol. 6; no. 7; pp. 1053 - 1056
• Main Authors: Shabala, Sergey, Bækgaard, Lone, Shabala, Lana, Fuglsang, Anja T., Cuin, Tracey Ann, Nemchinov, Lev G., Palmgren, Michael G.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.07.2011; Landes Bioscience
• Subjects: Addendum; antiporters; Binding; Biology; Bioscience; Ca2-transporting ATPase; Calcium; Calcium - metabolism; calcium channels; Calcium-Transporting ATPases - genetics; Calcium-Transporting ATPases - metabolism; Cancer; Cell; Cell Membrane - metabolism; Cycle; cytosol; electrophysiology; environmental factors; genomics; Landes; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Organogenesis; oxidative stress; Oxidative Stress - genetics; Oxidative Stress - physiology; plasma membrane; Potato virus X; Potexvirus - physiology; Proteins; protons; Signal Transduction - genetics; Signal Transduction - physiology
• ISSN: 1559-2316; 1559-2324; 1559-2324
• DOI: 10.4161/psb.6.7.15634

Although the role of Ca 2+ influx channels in oxidative stress signaling and cross-tolerance in plants is well established, little is known about the role of active Ca 2+ efflux systems in this process. In our recent paper, 17 we reported Potato Virus X (PVX)-induced acquired resistance to oxidative stress in Nicotiana benthamiana and showed the critical role of plasma membrane Ca 2+ /H + exchangers in this process. The current study continues this research. Using biochemical and electrophysiological approaches, we reveal that both endomembrane P 2A and P 2B Ca 2+ -ATPases play significant roles in adaptive responses to oxidative stress by removing excessive Ca 2+ from the cytosol, and that their functional expression is significantly altered in PVX-inoculated plants. These findings highlight the crucial role of Ca 2+ efflux systems in acquired tolerance to oxidative stress and open up prospects for practical applications in agriculture, after in-depth comprehension of the fundamental mechanisms involved in common responses to environmental factors at the genomic, cellular and organismal levels.