Effect of sodium acetate on the adhesion to porcine gastric mucin in a Lactococcus lactis strain grown on fructose

• Published in: Animal science journal Vol. 87; no. 6; pp. 802 - 808
• Main Authors: Kimoto-Nira, Hiromi, Moriya, Naoko, Yamasaki, Seishi, Takenaka, Akio, Suzuki, Chise
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.06.2016
• Subjects: adhesive activity; Animals; Bacteria; Bacterial Adhesion; Carbohydrate Metabolism; Culture Media; Fatty Acids - metabolism; fructose; Fructose - pharmacology; Gastric Mucins - metabolism; Gastrointestinal Tract - microbiology; Humans; Ions; Lactococcus lactis; Lactococcus lactis - growth & development; Lactococcus lactis - metabolism; Lactococcus lactis - physiology; Pork; Probiotics; Sodium; sodium acetate; Sodium Acetate - pharmacology; Swine; Vinegar; adhesive activity; fructose; sodium acetate; probiotics
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12487

The association of lactic acid bacteria with mucosal surfaces plays important roles in the beneficial effects of these bacteria on human health, such as colonization of the gastrointestinal tract for pathogen antagonism. Previously, we found that the adhesion of Lactococcus lactis 7‐1 to porcine gastric mucin was higher with fructose than with lactose, galactose or xylose as the carbon source. In this study, we examined the effect of growth conditions on the adhesion of strain 7‐1 grown on fructose. Medium components affect the adhesion: the adhesion of strain 7‐1 grown with sodium acetate was higher than that without it. The enhancement of adhesion by sodium acetate was not observed under aerobic conditions. Cellular properties grown with or without sodium acetate were characterized: strain 7‐1 grown with sodium acetate had similar sugar contents, and different fatty acid composition to those grown without it. Strain 7‐1 grown with sodium acetate showed significantly lower cell yield and significantly higher hydrophobicity than those grown without it, which is associated with higher adhesion. Fructose and sodium acetate are frequently used in the food industry; this study may reveal a simple way to enhance the adhesion of lactic acid bacteria by growing them with these substances.