From 3D to 2D: A Review of the Molecular Imprinting of Proteins
Molecular imprinting is a generic technology that allows for the introduction of sites of specific molecular affinity into otherwise homogeneous polymeric matrices. Commonly this technique has been shown to be effective when targeting small molecules of molecular weight <1500, while extending the...
Saved in:
Published in | Biotechnology progress Vol. 22; no. 6; pp. 1474 - 1489 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
USA
American Chemical Society
01.12.2006
|
Online Access | Get full text |
Cover
Loading…
Summary: | Molecular imprinting is a generic technology that allows for the introduction of sites of specific molecular affinity into otherwise homogeneous polymeric matrices. Commonly this technique has been shown to be effective when targeting small molecules of molecular weight <1500, while extending the technique to larger molecules such as proteins has proven difficult. A number of key inherent problems in protein imprinting have been identified, including permanent entrapment, poor mass transfer, denaturation, and heterogeneity in binding pocket affinity, which have been addressed using a variety of approaches. This review focuses on protein imprinting in its various forms, ranging from conventional bulk techniques to novel thin film and monolayer surface imprinting approaches. |
---|---|
Bibliography: | ark:/67375/WNG-22Z4RFWF-H istex:E0EABCA1179296AAF2FC328F465A9B347ABF1628 ArticleID:BTPR60122 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 8756-7938 1520-6033 |
DOI: | 10.1021/bp060122g |