Evaluation of the accuracy of the microplate Alamar Blue assay for rapid detection of MDR-TB in Peru [Short Communication]

Tuberculosis control is hampered by the widespread increase in multidrug resistance. Rapid drug susceptibility testing would greatly aid in the adequate treatment of the disease. This study evaluates the usefulness of the colorimetric method using Alamar Blue for the rapid detection of resistance to...

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Bibliographic Details
Published inThe international journal of tuberculosis and lung disease Vol. 11; no. 7; pp. 820 - 822
Main Authors CHAUCA, J. A, PALOMINO, J.-C, GUERRA, H
Format Journal Article
LanguageEnglish
Published Paris, France IUATLD 01.07.2007
Union internationale contre la tuberculose et les maladies respiratoires
Subjects
Accuracy
Antitubercular Agents - administration & dosage
Bacterial diseases
Bacterial Typing Techniques
Biological and medical sciences
Colorimetry - methods
Drug Resistance
Drug Resistance, Bacterial
Female
Human bacterial diseases
Humans
Infectious diseases
Maba
Male
Medical sciences
Microbial Sensitivity Tests
Mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - isolation & purification
Peru
Pneumology
Reagent Kits, Diagnostic
Sampling Studies
Sensitivity and Specificity
Time Factors
Tuberculosis
Tuberculosis and atypical mycobacterial infections
Tuberculosis, Multidrug-Resistant - diagnosis
Tuberculosis, Multidrug-Resistant - drug therapy
South America
Peru
America
Evaluation
Treatment resistance
Drug susceptibility test
Mycobacterial infection
Infection
MABA
Antibiotic
Accuracy
Rapid technique
Rifampicin
Tuberculosis
Multiple resistance
Bacteriosis
Antituberculous agent
drug resistance
Antibacterial agent
Isoniazid
Pneumology
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Summary:Tuberculosis control is hampered by the widespread increase in multidrug resistance. Rapid drug susceptibility testing would greatly aid in the adequate treatment of the disease. This study evaluates the usefulness of the colorimetric method using Alamar Blue for the rapid detection of resistance to rifampicin and isoniazid in 63 clinical isolates of Mycobacterium tuberculosis in Peru. Results obtained by receiver operating characteristic curve analysis and measures of gain in certainty showed greater diagnostic accuracy than with the gold standard, the proportion method on Löwenstein-Jensen medium.
Bibliography:1027-3719(20070701)11:7L.820;1-
(R) Medicine - General
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ISSN:1027-3719
1815-7920