Histological analysis and gene expression profile following augmentation of the anterior maxilla using rhBMP-2/ACS versus autogenous bone graft
Aim The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant human bone morphogenetic protein‐2 in an absorbable collagen sponge carrier (rhBMP‐2/ACS)...
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Published in | Journal of clinical periodontology Vol. 43; no. 12; pp. 1200 - 1207 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.12.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Aim
The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant human bone morphogenetic protein‐2 in an absorbable collagen sponge carrier (rhBMP‐2/ACS) versus an autogenous bone graft (ABG).
Methods
Bone core biopsies from 24 subjects participating in a randomized clinical trial were obtained at dental implant placement, 6 months following alveolar ridge augmentation using rhBMP‐2/ACS (rhBMP‐2 at 1.5 mg/ml; total dose 4.2 mg) or a particulate ABG harvested from the mandibular retro‐molar region. A titanium mesh was used to provide wound stability and space for bone formation. Analysis included histological/histometric observations and gene expression profile of the newly formed bone.
Results
rhBMP‐2/ACS yielded bone marrow rich in capillaries, undifferentiated cells and bone lining cells compared with the ABG (p = 0.002). Whereas no significant differences were observed in total bone fraction (p = 0.53), non‐vital bone particles trapped in lamellar vital bone were observed in the ABG group (p < 0.001). Real‐time PCR showed greater BMP‐2 and RUNX2 expression for rhBMP‐2/ACS over the ABG (p = 0.001 and 0.0021, respectively), while the ABG exhibited greater expression of RANKL:OPG, BSP and OPN over rhBMP‐2/ACS (p = 0.01, 0.005 and 0.0009, respectively).
Conclusions
Our observations suggest that formative biological processes explain bone formation following implantation of rhBMP‐2/ACS, whereas remodelling, resorptive/formative processes, characterizes sites receiving ABGs. |
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Bibliography: | ArticleID:JCPE12601 istex:FA091F62ABA8CE81A29D76BC43CD62DE38AF4D9C Sao Paulo Research Foundation (FAPESP) - No. 2009/16016-8 CAPES Nobel Biocare ark:/67375/WNG-LT27HKN1-R Research funding was provided from the Sao Paulo Research Foundation (FAPESP) – (grant number 2009/16016‐8), São Paulo, Brazil and CAPES, Brasilia, Brazil. Drs. Wikesjö and Susin were supported in part by a grant from Nobel Biocare, Zürich, Switzerland. Conflict of interest and source of funding statement The authors declare no conflict of interest related to this study. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-News-1 ObjectType-Feature-3 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0303-6979 1600-051X |
DOI: | 10.1111/jcpe.12601 |