Expression of extracellular matrix metalloproteinase inducer glycosylation and caveolin-1 in healthy and inflamed human gingiva

• Published in: Journal of periodontal research Vol. 49; no. 2; pp. 197 - 204
• Main Authors: Wang, J., Yang, D., Li, C., Shang, S., Xiang, J.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.04.2014
• Subjects: Adolescent; Adult; Basigin - analysis; Caveolin 1 - analysis; Caveolin-1; Chronic Periodontitis - metabolism; Chronic Periodontitis - pathology; Dental Plaque Index; EMMPRIN; Endothelial Cells - chemistry; Endothelium, Vascular - chemistry; Epithelial Cells - chemistry; Female; Fibroblasts - chemistry; Gingiva - chemistry; Gingiva - cytology; Glycosylation; Humans; Male; Matrix Metalloproteinase 1 - analysis; Middle Aged; MMP-1; Periodontal Attachment Loss - classification; Periodontal Index; Periodontal Pocket - classification; periodontitis; Young Adult; Caveolin-1; EMMPRIN; glycosylation; MMP-1; periodontitis
• ISSN: 0022-3484; 1600-0765; 1600-0765
• DOI: 10.1111/jre.12095

Background and Objective Glycosylated extracellular matrix metalloproteinase inducer (EMMPRIN) is specifically associated with caveolin‐1 and influences its ability to induce matrix metalloproteinases (MMPs) production. This study investigated EMMPRIN glycosylation and caveolin‐1 expression in healthy and inflamed human gingival tissues, analyzed the relationship between EMMPRIN glycosylation and caveolin‐1 expression, and assessed how this interaction influenced MMP‐1 production. Material and Methods Gingival tissues were collected from 10 healthy subjects and 15 chronic periodontitis (chronic periodontitis) subjects. EMMPRIN, caveolin‐1 and MMP‐1 expressions were analyzed by immunohistochemistry. EMMPRIN and caveolin‐1 co‐localization was detected by immunofluorescence. EMMPRIN glycosylation, caveolin‐1, active MMP‐1 and proMMP‐1 expression was assessed by Western blot. Results EMMPRIN was expressed in gingival epithelial cells, inflammatory cells, endothelial and fibroblast‐like cells. Strong caveolin‐1 immunoreactivity was detected in gingival epithelial and endothelial cells. Double immunofluorescence studies revealed EMMPRIN and caveolin‐1 co‐localization in gingival epithelium, endothelial and fibroblast‐like cells. Compared with healthy subjects, the chronic periodontitis group had increased high‐glycoform EMMPRIN (HG‐EMMPRIN) and active MMP‐1 expression (p < 0.05). Active MMP‐1 and proMMP‐1 protein levels were positively correlated with HG‐EMMPRIN levels (p < 0.05). Conclusion EMMPRIN and caveolin‐1 colocalize in periodontal tissues. The increased active MMP‐1 and proMMP‐1 production may be associated with elevated HG‐EMMPRIN levels.