Synthesis of Arylazide- and Diazirine-Containing CrAsH-EDT2 Photoaffinity Probes
Two photo‐crosslinking biarsenical (CrAsH‐EDT2)‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of...
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Published in | Archiv der Pharmazie (Weinheim) Vol. 349; no. 4; pp. 233 - 241 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English German |
Published |
WEINHEIM
Blackwell Publishing Ltd
01.04.2016
Wiley Wiley Subscription Services, Inc John Wiley and Sons Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Two photo‐crosslinking biarsenical (CrAsH‐EDT2)‐modified probes were synthesized that are expected to be useful tools for tetracysteine‐labeled proteins to facilitate the co‐affinity purification of their DNA binding sequences and interacting proteins. In addition, improvements for the synthesis of CrAsH‐EDT2 and N1‐(4‐azido‐2‐nitrophenyl)hexane‐1,6‐diamine are reported. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 (doublesex and Mab3‐related transcription factor) to induce fluorescence, suggesting that their crosslinking abilities can be exploited for the identification of nucleic acids and proteins associated with a protein of interest.
Two photo‐crosslinking biarsenical probes were synthesized that are expected to be useful tools for identifying the DNA binding sequences and interacting proteins of tetracysteine‐labeled proteins, by co‐affinity purification. Both photoprobes effectively entered HeLa cells (and the nucleus) and were dependent on the tetracysteine motif in recombinant DMRT1 to induce fluorescence. |
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Bibliography: | ark:/67375/WNG-XF8ND8WZ-3 istex:22ED68AB959D409571CBCB202370D9A9511E09D3 ArticleID:ARDP201500440 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0365-6233 1521-4184 |
DOI: | 10.1002/ardp.201500440 |