Archaebacterial ATPases: Relationship to Other Ion-Translocating ATPase Families Examined in Terms of Immunological Cross-Reactivity

• Published in: Journal of biochemistry (Tokyo) Vol. 108; no. 4; pp. 554 - 559
• Main Authors: Konishi, Jin, Denda, Kimitoshi, Oshima, Tairo, Wakagi, Takayoshi, Uchida, Etsuko, Ohsumi, Yoshinori, Anraku, Yasuhiro, Matsumoto, Takao, Wakabayashi, Takeyuki, Mukohata, Yasuo, Ihara, Kunio, Ken-ichi, Inatomi, Kato, Keitaro, Ohta, Toshiko, William, S. Allison, Yoshida, Masasuke
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.10.1990
• Subjects: Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - immunology; adenosinetriphosphatase; antibodies; Archaea - enzymology; Archaea - genetics; Archaea - immunology; archebacteria; Biological Evolution; Biological Transport; Cross Reactions; Electrophoresis, Polyacrylamide Gel; Exact sciences and technology; Gram-Negative Chemolithotrophic Bacteria - enzymology; Gram-Negative Chemolithotrophic Bacteria - genetics; Gram-Negative Chemolithotrophic Bacteria - immunology; H super(+)-transporting ATPase; Mathematical analysis; Mathematics; Potential theory; Proton-Translocating ATPases - genetics; Proton-Translocating ATPases - immunology; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - immunology; Sciences and techniques of general use; Sodium-Potassium-Exchanging ATPase - genetics; Sodium-Potassium-Exchanging ATPase - immunology; Vacuoles - enzymology; Vacuoles - immunology; Yeast; Archaeobacteria; Gel electrophoresis; Enzyme; Biological evolution; F0F1-ATPase; Electron microscopy; F1-»ATPase; Fungi; Na,K-»ATPase; Cross reaction; Immunoblotting assay; Bacteria; Ascomycetes; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a123241

Immunological cross-reactivity among three types of H+-ATPases, that is, three archaebacterial ATPases, the F1-ATPase from thermophilic bacterium PS3 (TF1 and the vacuolar membrane ATPase from Saccharomyces cerevisiae, was examined by means of immunoblot analyses. The three archaebacterial ATPases were very similar in immunological cross-reactivity, suggesting that they belong to the same family of ATPases. Cross-reaction was also observed between the ATPase from Sulfolobus acidocaldarius, one of the three archaebacteria, and TF1. S. cerevisiae vacuolar ATPase reacted with the antibodies prepared against each of the three archaebacterial ATPases, but did not react with the antibody against TF1. Electron microscopic examination revealed that the oligomeric structure of Sulfolobus ATPase was very similar to that of F1 -ATPase. These results, taken together, suggest that the archaebacterial ATPases share close structural similarities with the vacuolar ATPases, and, to a lesser degree, with the F0F1-ATPases.