Poly-N-Acetyllactosamines Synthesized by Cultured Ehrlich Carcinoma Cells: Application of Endo-β-Galactosidase C for Analysis of the Terminal Structure

• Published in: Journal of biochemistry (Tokyo) Vol. 104; no. 5; pp. 738 - 741
• Main Authors: Kamada, Yuko, Muramatsu, Hisako, Kawata, Makoto, Takamizawa, Hiroyoshi, Muramatsu, Takashi
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.1988
• Subjects: Analytical, structural and metabolic biochemistry; Animals; beta-Galactosidase - metabolism; Biological and medical sciences; Carbohydrates; Carbon Radioisotopes; Carcinoma, Ehrlich Tumor - metabolism; Chromatography, Gel; Chromatography, Thin Layer; Fundamental and applied biological sciences. Psychology; Galactosidases - metabolism; Glycoside Hydrolases; Methylation; Other biological molecules; poly-N-acteyllactosamines; Polysaccharides - analysis; Polysaccharides - biosynthesis; Cell culture; Carcinoma; Investigation method; Tumor; Cell transformation; Ehrlich ascite cell; Polysaccharide; Cell surface

Poly-N-acetyllactosamines were prepared from Ehrlich carcinoma cells cultured in the presence of [14C] galactose. Methylation analysis indicated that 31% of the galactose was in the non-reducing end. Of it, 77% was cleaved by α-galactosidase, and 56% was released as a disaccharide by endo-β-galactos...