Poly-N-Acetyllactosamines Synthesized by Cultured Ehrlich Carcinoma Cells: Application of Endo-β-Galactosidase C for Analysis of the Terminal Structure

• Published in: Journal of biochemistry (Tokyo) Vol. 104; no. 5; pp. 738 - 741
• Main Authors: Kamada, Yuko, Muramatsu, Hisako, Kawata, Makoto, Takamizawa, Hiroyoshi, Muramatsu, Takashi
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.1988
• Subjects: Analytical, structural and metabolic biochemistry; Animals; beta-Galactosidase - metabolism; Biological and medical sciences; Carbohydrates; Carbon Radioisotopes; Carcinoma, Ehrlich Tumor - metabolism; Chromatography, Gel; Chromatography, Thin Layer; Fundamental and applied biological sciences. Psychology; Galactosidases - metabolism; Glycoside Hydrolases; Methylation; Other biological molecules; poly-N-acteyllactosamines; Polysaccharides - analysis; Polysaccharides - biosynthesis; Cell culture; Carcinoma; Investigation method; Tumor; Cell transformation; Ehrlich ascite cell; Polysaccharide; Cell surface
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a122543

Poly-N-acetyllactosamines were prepared from Ehrlich carcinoma cells cultured in the presence of [14C] galactose. Methylation analysis indicated that 31% of the galactose was in the non-reducing end. Of it, 77% was cleaved by α-galactosidase, and 56% was released as a disaccharide by endo-β-galactosidase C. Methylation analysis confirmed that the released disaccharide was mostly Gal α1→3Gal. Therefore, Gal α1→3Gal structure, not Galα1→3(Galα1→6)Gal structure, was the major α-galactosyl structure in the poly-N-acetyllactosamines synthesized. Furthermore, α-galactosidase digestion did not change the content of disubstituted galactosyl residues. Thus, Galα1→3(Galα1→6)Gal structure, which was suggested to be the sole non-reducing terminal structure of poly-N-acetyllactosamines of Ehrlich carcinoma cells, was not detected in significant amounts under the present experimental conditions.