Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population
Aim To examine the genetic diversity of Aggregatibacter actinomycetemcomitans in Thai adults. Materials and Methods Subgingival plaque samples from 453 subjects were analysed for A. actinomycetemcomitans serotypes, the presence of the high leukotoxin‐producing JP2 clone and cytolethal distending tox...
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Published in | Journal of clinical periodontology Vol. 39; no. 6; pp. 519 - 525 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Blackwell Publishing Ltd
01.06.2012
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | Aim
To examine the genetic diversity of Aggregatibacter actinomycetemcomitans in Thai adults.
Materials and Methods
Subgingival plaque samples from 453 subjects were analysed for A. actinomycetemcomitans serotypes, the presence of the high leukotoxin‐producing JP2 clone and cytolethal distending toxin genes (cdtABC) using the polymerase chain reaction technique. In subjects who were positive for cdtABC, restriction fragment length polymorphism analysis was used to identify a single nucleotide polymorphism (SNP) in the cdtB gene at amino acid position 281. The extent and severity of periodontal disease were compared between subjects harbouring different A. actinomycetemcomitans genotypes.
Results
Eighty six subjects (19%) were positive for A. actinomycetemcomitans. The JP2 clone was not detected. Serotype c was the most prevalent (57%), followed by serotypes a (33%) and b (7%). Among A. actinomycetemcomitans‐positive subjects, 27% were positive for cdtABC. All cdtABC‐positive subjects possessed the SNP in the cdtB, which is involved with increased toxin activity. The presence of A. actinomycetemcomitans, but not a specific genotype, was significantly related to increased probing depth and periodontal attachment loss.
Conclusions
Our results confirm the previous findings that genotype distribution of A. actinomycetemcomitans varies between ethnic groups. However, no clear relationship between a specific genotype and periodontal conditions was observed. |
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Bibliography: | Faculty of Dentistry, Chulalongkorn University Research Fund - No. DRF 52001; No. DRF 53018 ark:/67375/WNG-VTC6HM22-J National Research University Development Project for Higher Education Research Promotion istex:96AB599B36AD154D789E90E1BA03E4C30EB3C5B4 Office of the Higher Education Commission ArticleID:JCPE1871 The authors declare that they have no conflict of interests. This work was supported by the Faculty of Dentistry, Chulalongkorn University Research Fund (DRF 52001 and DRF 53018), and the Project for Higher Education Research Promotion and National Research University Development, Office of the Higher Education Commission. Conflict of interest and source of funding statement ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0303-6979 1600-051X |
DOI: | 10.1111/j.1600-051X.2012.01871.x |