Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila
Posttranscriptional gene silencing (PTGS) induced by double‐stranded RNA (dsRNA) is an intriguing phenomenon that has been observed in a variety of organisms, including Drosophila melanogaster. Although PTGS in Drosophila is typically observed following direct injection of the dsRNA into embryos, it...
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Published in | Genesis (New York, N.Y. : 2000) Vol. 26; no. 4; pp. 240 - 244 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
01.04.2000
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Subjects | |
Online Access | Get full text |
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Summary: | Posttranscriptional gene silencing (PTGS) induced by double‐stranded RNA (dsRNA) is an intriguing phenomenon that has been observed in a variety of organisms, including Drosophila melanogaster. Although PTGS in Drosophila is typically observed following direct injection of the dsRNA into embryos, it is theoretically possible that the in vivo transcription of an inverted repeat transgene might also produce a dsRNA “hairpin” that is capable of triggering PTGS. Here we test this idea, and show that an expressed inverted repeat of a portion of the sex differentiation gene, transformer‐2, (tra‐2), driven by a GAL4‐dependent promoter, does genetically repress the endogenous wild‐type tra‐2 function, producing a dominant loss‐of‐function mutant phenotype. Remarkably, this effect is temperature‐sensitive, with phenotypic consequences seen at 29°, but not at 22°. Moreover, by altering the dosage of either the transgenes or the endogenous tra2+ loci, one can vary the effect over a wide range of mutant phenotypes. genesis 26:240–244, 2000. © 2000 Wiley‐Liss, Inc. |
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Bibliography: | ArticleID:GENE40 National Science Foundation istex:79C9BE34014FD73F76D0BA8CA9A083961BA7FC27 ark:/67375/WNG-PTVKBMQ2-R ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1526-954X 1526-968X |
DOI: | 10.1002/(SICI)1526-968X(200004)26:4<240::AID-GENE40>3.0.CO;2-P |