Oridonin's therapeutic effect: Suppressing Th1/Th17 simultaneously in a mouse model of Crohn's disease

• Published in: Journal of gastroenterology and hepatology Vol. 30; no. 3; pp. 504 - 512
• Main Authors: Wang, Shubei, Zhang, Yong, Saas, Philippe, Wang, Haili, Xu, Ying, Chen, Ke, Zhong, Jie, Yuan, Yaozong, Wang, Ying, Sun, Yunwei
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01.03.2015
• Subjects: animal models; Animals; Apoptosis - drug effects; Cells, Cultured; Crohn Disease - chemically induced; Crohn Disease - drug therapy; Crohn Disease - immunology; cytokines; Disease Models, Animal; Diterpenes, Kaurane - pharmacology; Diterpenes, Kaurane - therapeutic use; Interferon-gamma - metabolism; Interleukin-17 - metabolism; Male; Mice, Inbred BALB C; NF-kappa B - genetics; oridonin; Phytotherapy; Spleen - cytology; Spleen - immunology; Th1 Cells - immunology; Th17 Cells - immunology; Trinitrobenzenesulfonic Acid; Up-Regulation - drug effects; oridonin; animal models; cytokines
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1111/jgh.12710

Background and Aim Crohn's disease is a chronic inflammatory bowel disease. Oridonin is an effective component isolated from Rabdosia rubescens. It can inhibit the activation of transcription factor nuclear factor‐kappa B and suppress the over expression of cytokines. We postulated that oridonin may be a potential therapeutic candidate for Crohn's disease. Methods To confirm the postulation, we investigated clinical and immunologic modulations of oridonin in a mouse model of trinitrobenzene sulfonic acid‐induced colitis. Results It was found that oridonin attenuated trinitrobenzene sulfonic acid‐induced colitis as represented by a reduction in colonic interferon‐γ/inteleukin‐17 secretion and a decrement in splenic Th1/Th17 cells and effector memory CD4+ T cells. Oridonin treatment inhibited the proliferation of CD4+ T cells and upregulated the apoptosis of lymphocytes by inhibiting nuclear translocation of transcription factor nuclear factor‐kappa B. Conclusions Oridonin is a potential modulator for trinitrobenzene sulfonic acid‐induced colitis and other Th1/Th17 mediated inflammatory diseases.