Transcriptional suppression of Sox9 expression in chondrocytes by retinoic acid
SOX9 is a transcription factor that is expressed in chondrocytes and regulates expression of chondrocyte phenotype related genes. Expression of these genes is known to be suppressed by retinoic acid (RA). We, therefore, examined whether the Sox9 gene expression is regulated by RA in chondrocytes. RA...
Saved in:
Published in | Journal of cellular biochemistry Vol. 81; no. S36; pp. 71 - 78 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
2001
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | SOX9 is a transcription factor that is expressed in chondrocytes and regulates expression of chondrocyte phenotype related genes. Expression of these genes is known to be suppressed by retinoic acid (RA). We, therefore, examined whether the Sox9 gene expression is regulated by RA in chondrocytes. RA treatment suppressed Sox9 mRNA expression in primary chondrocytes prepared from newborn mouse rib cartilage within 12 h and this suppression lasted at least up to 24 h. The RA suppression of Sox9 mRNA levels was dose‐dependent starting at 0.5 μM with a maximum at 1 μM. Nuclear run‐on assays revealed that RA reduced the rate of transcription of Sox9 gene. Finally, Western blot analysis indicated that RA suppressed SOX9 protein levels in these chondrocytes. Furthermore, overexpression of SOX9 reversed RA suppression of Col2a1 enhancer activity. These observations indicate that RA suppresses Sox9 gene expression in chondrocytes at least in part through transcriptional events. J. Cell. Biochem. Suppl. 36: 71–78, 2001. © 2001 Wiley‐Liss, Inc. |
---|---|
Bibliography: | istex:D0E199DFA722D3D1D82E53EA3E39925603234761 ArticleID:JCB1077 "Research for the Future" Program of the Japan Society for the Promotion of Science(JSPS) - No. 96100205 Inamori Foundation Interdisciplinary Cancer Research Foundation Traffic Medicine Foundation NASDA to MN ark:/67375/WNG-9VPGS7Z2-F Japanese Ministry of Education - No. 11877357; No. 10044246; No. 10877223; No. 0930734 CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation (JST) |
ISSN: | 0730-2312 0733-1959 1097-4644 |
DOI: | 10.1002/jcb.1077 |