Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris

• Published in: Biotechnology and applied biochemistry Vol. 62; no. 1; p. 64
• Main Authors: Li, Ren-Kuan, Chen, Ping, Ng, Tzi Bun, Yang, Jie, Lin, Juan, Yan, Fen, Ye, Xiu-Yun
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Amorphophallus - chemistry; Bacillus subtilis - enzymology; Bacillus subtilis - genetics; beta-Mannosidase - biosynthesis; beta-Mannosidase - genetics; beta-Mannosidase - isolation & purification; beta-Mannosidase - metabolism; Cloning, Molecular; Flour; Gene Expression; Genetic Engineering - methods; Hydrogen-Ion Concentration; Hydrolysis; Pichia - genetics; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Substrate Specificity; Temperature; β-mannanase; highly efficient expression; Pichia pastoris; Bacillus subtilis
• ISSN: 1470-8744
• DOI: 10.1002/bab.1250

A β-mannanase gene (Man5) from Bacillus subtilis BS5 was cloned by PCR and integrated into the genome of Pichia pastoris GS115 via pPIC9 vector. The recombinant Man5 with a molecular mass of 43 kDa was successfully expressed and secreted into the culture medium. After methanol induction in a shake flask for 96 H, the recombinant Man5 protein reached 375 µg/mL in concentration, with an enzyme activity of 892 U/mL. The recombinant Man5 was purified 3.35-fold with 60% yield by using HiTrap DEAE FF and HiTrap Phenyl FF columns. The specific activity of the purified enzyme was 7,978 U/mg. The optimum temperature and pH of the recombinant Man5 were 50 °C and 6.0, respectively. Studies of substrate specificity showed that the optimum substrate for the Man5 was konjac flour, suggesting that it has great potential as an effective additive in the food industry.