Collection of Peripheral Blood CD34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a Baxter-Fenwal CS-3000 Plus Blood Cell Separator
Background Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter‐Fenwal CS‐3000 Plus automated blood cell separator has not been reported. Objective To determine the feasibility and safety of using a CS‐3000 Plus blood cell separator with a small volume separation container holder...
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Published in | Journal of veterinary internal medicine Vol. 25; no. 6; pp. 1406 - 1413 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.11.2011
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Subjects | |
Online Access | Get full text |
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Summary: | Background
Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter‐Fenwal CS‐3000 Plus automated blood cell separator has not been reported.
Objective
To determine the feasibility and safety of using a CS‐3000 Plus blood cell separator with a small volume separation container holder (SVSCH) and small volume collection chamber (SVCC) to harvest canine PBMCs from dogs weighing <50 kg.
Animals
Eight healthy mongrel dogs and 11 client‐owned dogs in clinical remission for lymphoproliferative diseases (LPD).
Methods
In this prospective study, aphereses were performed using a Baxter‐Fenwal CS‐3000 Plus blood cell separator, with or without recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) treatment.
Results
Aphereses from 6 healthy dogs given rhG‐CSF yielded an average of 1.1 × 107 ± 8.2 × 106 CD34+ cells/kg. Aphereses from LPD dogs given rhG‐CSF yielded an average of 5.4 × 106 ± 3.25 × 106 CD34+ cells/kg (P = .17). Higher hematocrit in both groups of dogs receiving rhG‐CSF correlated with an increased number of CD34+ cells/kg harvested (healthy, P = .04; LPD, P = .05). Apheresis was well tolerated by all dogs.
Conclusions and Clinical Importance
Canine PBMC apheresis using the Baxter‐Fenwal CS‐3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation. |
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Bibliography: | istex:DE1137236961DA590E6D4D90772EBDE267BE5C84 ark:/67375/WNG-M0LCW4CR-K ArticleID:JVIM827 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0891-6640 1939-1676 |
DOI: | 10.1111/j.1939-1676.2011.00827.x |