Collection of Peripheral Blood CD34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a Baxter-Fenwal CS-3000 Plus Blood Cell Separator

• Published in: Journal of veterinary internal medicine Vol. 25; no. 6; pp. 1406 - 1413
• Main Author: Suter, S.E.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.11.2011
• Subjects: Animals; Antigens, CD34 - metabolism; Apheresis; Canine; Cell Separation - instrumentation; Cell Separation - methods; Cell Separation - veterinary; Dog Diseases - pathology; Dogs; Female; Hematopoietic cell transplantation; Hematopoietic Stem Cells - metabolism; Leukapheresis - instrumentation; Leukapheresis - veterinary; Lymphoproliferative Disorders - blood; Lymphoproliferative Disorders - metabolism; Lymphoproliferative Disorders - veterinary; Male; Mononuclear cells; Reproducibility of Results
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/j.1939-1676.2011.00827.x

Background Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter‐Fenwal CS‐3000 Plus automated blood cell separator has not been reported. Objective To determine the feasibility and safety of using a CS‐3000 Plus blood cell separator with a small volume separation container holder (SVSCH) and small volume collection chamber (SVCC) to harvest canine PBMCs from dogs weighing <50 kg. Animals Eight healthy mongrel dogs and 11 client‐owned dogs in clinical remission for lymphoproliferative diseases (LPD). Methods In this prospective study, aphereses were performed using a Baxter‐Fenwal CS‐3000 Plus blood cell separator, with or without recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) treatment. Results Aphereses from 6 healthy dogs given rhG‐CSF yielded an average of 1.1 × 107 ± 8.2 × 106 CD34+ cells/kg. Aphereses from LPD dogs given rhG‐CSF yielded an average of 5.4 × 106 ± 3.25 × 106 CD34+ cells/kg (P = .17). Higher hematocrit in both groups of dogs receiving rhG‐CSF correlated with an increased number of CD34+ cells/kg harvested (healthy, P = .04; LPD, P = .05). Apheresis was well tolerated by all dogs. Conclusions and Clinical Importance Canine PBMC apheresis using the Baxter‐Fenwal CS‐3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation.