Determination of formoterol in rat plasma by liquid chromatography-electrospray ionisation mass spectrometry
A sensitive method for the determination of formoterol in rat plasma is described, using high performance liquid chromatographic separation with tandem mass spectrometry. Samples were purified using liquid-liquid extraction and separated on CAPCELL PAK C18 UG120 (2.0 × 150 mm) with a mobile phase co...
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Published in | Pharmazie Vol. 62; no. 2; pp. 94 - 95 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Govi-Verlag
01.02.2007
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Subjects | |
Online Access | Get full text |
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Summary: | A sensitive method for the determination of formoterol in rat plasma is described, using high performance liquid chromatographic separation with tandem mass spectrometry. Samples were purified using liquid-liquid extraction and separated on CAPCELL PAK C18 UG120 (2.0 × 150 mm)
with a mobile phase consisting of a mixture of methanol- 50 mM ammonium hydrogen carbonate (1 : 1 v/v). Detection was performed with a TSQ 7000 mass spectrometer using positive ion electrospray ionisation, monitoring the shift from precursor ions for formoterol at m/z 344.9 to product ions
of m/z 121.0. The limit of quantitation of the method was found to be 0.1 ng/ml, when using 0.1 ml plasma. Plasma concentrations of formoterol could be quantified from 0.15 to 7.01 ng/ml, allowing the analysis of samples up to 32 h after a single oral dose of formoterol fumarate (0.25 mg)
to rats. |
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Bibliography: | 0031-7144(20070201)62:2L.94;1- ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0031-7144 |
DOI: | 10.1691/ph.2007.2.6134 |