Evaluation of Glucoiberin Reference Material from Iberis amara by Spectroscopic Fingerprinting

• Published in: Journal of natural products (Washington, D.C.) Vol. 65; no. 4; pp. 517 - 522
• Main Authors: JAKI, Birgit, STICHER, Otto, VEIT, Markus, FRöHLICH, Roland, PAULI, Guido F.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.04.2002; Glendale, AZ American Society of Pharmacognosy
• Subjects: Biological and medical sciences; Choline - analogs & derivatives; Choline - chemistry; Crystallography, X-Ray; General pharmacology; Glucosinolates - chemistry; Glucosinolates - isolation & purification; Glucosinolates - pharmacology; Glucosinolates - standards; Mass Spectrometry; Medical sciences; Molecular Conformation; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plants, Medicinal - chemistry; Reference Standards; Seeds - chemistry; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Validation; Chemical analysis; Glucosinolate; Seeds; Purity control; NMR spectrometry; X ray diffraction; Medicinal plant; Cruciferae; Dicotyledones; Antinutrient factor; Angiospermae; Plant origin; Thioglycoside; Goitrogen; Reference material; Isolation; Spermatophyta; Quantitative analysis; Structural analysis; Crystalline structure
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np0100800

Increasing worldwide regulations require increased efforts toward validation of analytical and pharmacological reference materials. A detailed survey of glucoiberin, a prototype lead constituent of therapeutic value, using 1D/2D NMR, MS, and X-ray spectroscopy provided precise phytochemical data for structure assignment. Quantitative reference validation was achieved by the recently proposed qNMR method.