Cryopreservation of In Vitro-Grown Shoot Tips of the Medicinal Species Cleome Spinosa (Cleomaceae) Applying Vitrification-Based Techniques

• Published in: Cryo-Letters Vol. 40; no. 4; p. 237
• Main Authors: Vilardo, A Frm, Mendonça, T F, Engelmann, F, Cordeiro, L S, Albarello, N, Simões-Gurgel, C
• Format: Journal Article
• Language: English
• Published: England 01.07.2019
• Subjects: Cleome; Cryopreservation - methods; Cryoprotective Agents; Plant Shoots; Sucrose; Vitrification
• ISSN: 0143-2044

Few cryopreservation studies have been reported with the genus Cleome. Due to the use of C. spinosa in traditional medicine and its valuable pharmacological potential, the long-term conservation of the species will allow the safe maintenance of its germplasm. This study compares two vitrification-based techniques on the cryopreservation of shoot tips of C. spinosa. The effect of sucrose preculture and different vitrification solutions was evaluated using vitrification and V Cryo-plate techniques. The supplementation of recovery medium with BAP was also assessed. The V Cryo-plate proved to be the most efficient technique. Treatment of shoot tips with PVS2 at 0°C resulted in a higher regeneration response after cryopreservation when compared to treatment with PVS2 and PVS3 at 25°C. The highest survival (83.3%) and recovery (76.6%) were achieved for shoot tips exposed to PVS2 for 90 min at 0°C and recovered on MS medium supplemented with 0.5 mg L BAP for 2 weeks. Plants regenerated from cryopreserved shoot tips maintained their in vitro multiplication capacity and showed a normal phenotypic aspect, demonstrating the efficiency of the cryopreservation protocol.