Optimization of Artificial Propagation in Piracanjuba Fish Brycon orbignyanus Using Cryopreserved Semen

BACKGROUND: Cryopreserved semen could facilitate procedures during the artificial reproduction in fish. Factors affecting cryopreservation efficiency are important to define efficient protocols. This study investigated the application of cryoprotectants on the quality of piracanjuba fish semen, the...

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Bibliographic Details
Published inCryo-Letters Vol. 37; no. 5; p. 330
Main Authors Felizardo, V O, Melo, C C V, Murgas, L D S, Andrade, E S, Navarro, R D, Ftreitas, T F
Format Journal Article
LanguageEnglish
Published England 01.09.2016
Subjects
Animals
Aquaculture - methods
Characidae - physiology
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Dimethyl Sulfoxide - pharmacology
Fertilization
Insemination, Artificial - methods
Insemination, Artificial - veterinary
Methanol - pharmacology
Semen - physiology
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
motility water
Fertilization
fish
gametes
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Summary:BACKGROUND: Cryopreserved semen could facilitate procedures during the artificial reproduction in fish. Factors affecting cryopreservation efficiency are important to define efficient protocols. This study investigated the application of cryoprotectants on the quality of piracanjuba fish semen, the sperm concentration required for oocyte fertilization and spermatic activation. We evaluated two intracellular cryoprotectant solutions (DMSO and methanol) and two extracellular cryoprotectant solutions (egg yolk and lactose) to cryopreserved piracanjuba semen. Sperm motility rate, motility duration and spermatic alterations were assessed. The protocol for piracanjuba semen cryopreservation can use solutions including either DMSO or methanol as intracellular cryoprotectant and egg yolk or lactose as extracellular cryoprotectants.
ISSN:0143-2044