A sensitive TLC method to identify Echinaceae pallidae radix

In this work a fast, simple and sensitive qualitative TLC method was developed to identify Echinaceae pallidae radix and to distinguish this drug from similar ones. The TLC method is based on the lipophilic compounds of E. pallida. Three mobile phases provided good separation, e.g. toluene/ethylacet...

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Bibliographic Details
Published inPharmazie Vol. 59; no. 8; pp. 608 - 611
Main Authors Schicke, B., Hagels, H., Freudenstein, J., Wätzig, H.
Format Journal Article
LanguageEnglish
Published Germany Govi-Verlag 01.08.2004
Subjects
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Echinacea - chemistry
Fluorescent Dyes
Plant Extracts - analysis
Plant Roots - chemistry
Solvents
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
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Summary:In this work a fast, simple and sensitive qualitative TLC method was developed to identify Echinaceae pallidae radix and to distinguish this drug from similar ones. The TLC method is based on the lipophilic compounds of E. pallida. Three mobile phases provided good separation, e.g. toluene/ethylacetate 7 + 3 (v/v). A marker substance was found which shows a blue fluorescence at an excitation wavelength of 366 nm after detection with a spray agent containing 95 volume parts ethanol 96%, 5 parts trifluoroacetic acid 99% and zinc ions in 0.15 molar concentration. After spraying the chromatogram was heated at 110 °C for 7 min. This method is superior to HPLC methods to characterise mixtures of Echinacea extracts in terms of selectivity due to this post-chromatographic derivatisation and subsequent fluorescence detection.
Bibliography:0031-7144(20040801)59:8L.608;1-
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ISSN:0031-7144