Extraction, separation and isolation of volatiles from Vitex agnus-castus L. (Verbenaceae) wild species of Sardinia, Italy, by supercritical CO2

Isolation of volatile concentrates from leaves, flowers and fruits of Vitex agnus-castus L. have been obtained by supercritical extraction with carbon dioxide. The composition of the volatile concentrates has been analysed by GC/MS. In all plant organs, the extracts are composed chiefly of α-pinene,...

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Published inNatural product research Vol. 24; no. 6; pp. 569 - 579
Main Authors Marongiu, Bruno, Piras, Alessandra, Porcedda, Silvia, Falconieri, Danilo, Gonçalves, Maria J., Salgueiro, Ligia, Maxia, Andrea, Lai, Roberta
Format Journal Article
LanguageEnglish
Published England Taylor & Francis Group 01.04.2010
Subjects
antifungal activity
Antifungal Agents - analysis
Antifungal Agents - pharmacology
Arthrodermataceae - drug effects
Epidermophyton floccosum
Flowers - chemistry
Fruit - chemistry
hydrodistillation
Italy
Microbial Sensitivity Tests
Microsporum canis
Oils, Volatile - chemistry
Oils, Volatile - pharmacology
Plant Leaves - chemistry
supercritical extraction
Trichophyton mentagrophytes
Verbenaceae
Vitex - chemistry
Vitex agnus-castus
Vitex agnus-castus L
Italy
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Summary:Isolation of volatile concentrates from leaves, flowers and fruits of Vitex agnus-castus L. have been obtained by supercritical extraction with carbon dioxide. The composition of the volatile concentrates has been analysed by GC/MS. In all plant organs, the extracts are composed chiefly of α-pinene, sabinene, 1,8-cineole, α-terpinyl acetate, (E)-caryophyllene, (E)-β-farnesene, bicyclogermacrene, spathulenol and manool. The main difference observed was in the content of sclarene, which was not present in the samples from flowers or fruits. To complete the investigation, a comparison with the hydrodistilled oil has been carried out. The minimal inhibitory concentration (MIC) and the minimal lethal concentration were used to evaluate the antifungal activity of the oils against dermatophyte strains (Trichophyton mentagrophytes, Microsporum canis, T. rubrum, M. gypseum and Epidermophyton floccosum). Antifungal activity of the leaf essential oil was the highest, with MIC values of 0.64 µL mL −1 for most of the strains.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786410902899915