Application of Phosphoenolpyruvate into Canine Red Blood Cell Cryopreservation with Hydroxyethyl Starch

• Published in: Cryo-Letters Vol. 26; no. 1; pp. 1 - 6
• Main Authors: Kim, Heejaung, Itamoto, Kazuhito, Une, Satoshi, Nakaichi, Munekazu, Taura, Yasuho, Sumida, Sajio
• Format: Journal Article
• Language: English
• Published: England Cryoletters 01.01.2005
• Subjects: 2,3-Diphosphoglycerate - metabolism; Animals; Blood Preservation - instrumentation; Blood Preservation - methods; Canine Red Blood Cells; Cryopreservation; Cryopreservation - instrumentation; Cryopreservation - methods; Cryoprotective Agents - metabolism; Cryoprotective Agents - pharmacology; Dogs; Erythrocytes - cytology; Erythrocytes - metabolism; Hydroxyethyl Starch; Hydroxyethyl Starch Derivatives - pharmacology; Osmotic Fragility; Phosphoenolpyruvate; Phosphoenolpyruvate - metabolism; Phosphoenolpyruvate - pharmacology
• ISSN: 0143-2044

Phosphoenolpyruvate (PEP) is a phosphorylated glycolytic intermediate that can penetrate the RBC membrane and be metabolized to 2,3-DPG and ATP. In this study, we evaluated the effects of PEP treatment on canine red blood cells (RBCs) cryopreserved with 12.5% (w/v) HES. RBCs were incubated for 30, 60, and 90 min at 37°C with PEP solution containing 60 mM mannitol, 30 mM sodium chloride, 25 mM glucose, 1 mM adenine and 50 mM PEP (340 mosm/kg), pH 6.0 and then cryopreserved in liquid nitrogen with 12.5% (w/v) HES for 2 weeks. 2,3-DPG and saline stabilities of the PEP treated groups were increased and osmotic fragility indices were significantly decreased compared to the untreated control group. There were no differences in 2,3-DPG levels within the PEP treated groups with different PEP incubation times. These results suggest that PEP treatment may be beneficial for the cryopreservation of canine RBCs with HES.