Field performance of sugarcane (Saccharum sp.) plants derived from cryopreserved calluses

This study compared the field performance of sugarcane plants originating from three different sources: control, non-cryopreserved embryogenic calluses, cryopreserved embryogenic calluses and macropropagated material of the same commercial hybrid. Several agronomic traits were evaluated on 100 plant...

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Bibliographic Details
Published inCryo-Letters Vol. 23; no. 1; p. 21
Main Authors Martínez-Montero, M E, Ojeda, E, Espinosa, A, Sánchez, M, Castillo, R, González-Arnao, M T, Engelmann, F, Lorenzo, J C
Format Journal Article
LanguageEnglish
Published England 01.01.2002
Subjects
Cryopreservation - methods
In Vitro Techniques
Plant Physiological Phenomena
Plant Stems - anatomy & histology
Poaceae - embryology
Seeds - growth & development
Time Factors
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Summary:This study compared the field performance of sugarcane plants originating from three different sources: control, non-cryopreserved embryogenic calluses, cryopreserved embryogenic calluses and macropropagated material of the same commercial hybrid. Several agronomic traits were evaluated on 100 plants per treatment over a 27-month period covering the growth of the stool and of the first ratoon. Significant differences between treatments were observed only during the first six months of field growth of sugarcane stools. Stems produced from in vitro cultured material, irrespective of their cryopreservation status, had a smaller diameter and a shorter height than those produced from macropropagated material. These differences disappeared by 12 months of stool field growth.
ISSN:0143-2044