Cryopreservation of Spermatozoa from Closed Colonies, and Inbred, Spontaneous Mutant, and Transgenic Strains of Rats

• Published in: Comparative medicine Vol. 53; no. 6; pp. 639 - 641
• Main Authors: Nakatsukasa, Ena, Kashiwazaki, Naomi, Takizawa, Akiko, Shino, Masao, Kitada, Kazuhiro, Serikawa, Tadao, Hakamata, Yoji, Kobayashi, Eiji, Takahashi, Riichi, Ueda, Masatsugu, Nakashima, Tatsuyuki, Nakagata, Naomi
• Format: Journal Article
• Language: English
• Published: United States American Association for Laboratory Animal Science 01.12.2003
• Subjects: Animals; Animals, Genetically Modified; Breeding; Cryopreservation - methods; Female; Fertility - physiology; Fetal Viability - physiology; Insemination, Artificial; Litter Size; Male; Pregnancy; Pregnancy Outcome; Rats; Rats, Inbred Strains; Rats, Mutant Strains; Semen Preservation; Sperm Motility - physiology; Spermatozoa
• ISSN: 1532-0820; 2769-819X

We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F344/DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at -196°C. After thawing at 37°C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.