Euthanasia Method for Mice in Rapid Time-Course Pulmonary Pharmacokinetic Studies
To develop a means of euthanasia to support rapid time-course pharmacokinetic studies in mice, we compared retroorbital and intravenous lateral tail vein injection of ketamine-xylazine with regard to preparation time, utility, tissue distribution, and time to onset of euthanasia. Tissue distribution...
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Published in | Journal of the American Association for Laboratory Animal Science Vol. 48; no. 5; pp. 506 - 511 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Association for Laboratory Animal Science
01.09.2009
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Subjects | |
Online Access | Get full text |
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Summary: | To develop a means of euthanasia to support rapid time-course pharmacokinetic studies in mice, we compared retroorbital and intravenous lateral tail vein injection of ketamine-xylazine with regard to preparation time, utility, tissue distribution, and time to onset of euthanasia.
Tissue distribution and time to onset of euthanasia did not differ between administration methods. However, retroorbital injection could be performed more rapidly than intravenous injection and was considered to be a technically simple and superior alternative for mouse euthanasia. Retroorbital
ketamine-xylazine, CO2 gas, and intraperitoneal pentobarbital then were compared as euthanasia agents in a rapid time-point pharmacokinetic study. Retroorbital ketamine-xylazine was the most efficient and consistent of the 3 methods, with an average time to death of
approximately 5 s after injection. In addition, euthanasia by retroorbital ketamine-xylazine enabled accurate sample collection at closely spaced time points and satisfied established criteria for acceptable euthanasia technique. |
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Bibliography: | 1559-6109(20090915)48:5L.506;1- |
ISSN: | 1559-6109 2769-6677 |