Molecular diagnosis using RNAscope in-situ hybridization in canine malignancies
Immunohistochemistry has been used extensively to evaluate protein expression in clinical and research settings. However, immunohistochemistry is not always successful in veterinary medicine due to the lack of reliable antibody options, poor tissue preservation, labor-intensive staining, and antigen...
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Published in | Canadian journal of veterinary research Vol. 84; no. 4; pp. 319 - 323 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Canada
Canadian Veterinary Medical Association
01.10.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Immunohistochemistry has been used extensively to evaluate protein expression in clinical and research settings. However, immunohistochemistry is not always successful in veterinary medicine due to the lack of reliable antibody options, poor tissue preservation, labor-intensive staining,
and antigen-retrieval optimization processes. RNAscope in-situ hybridization (ISH) is a powerful technology that uses a specific sequence probe to identify targeted mRNA. In this study, we demonstrate RNAscope ISH in 4 common canine malignancies, which are traditionally diagnosed by
histopathology and immunohistochemistry. Probes were designed for commonly targeted mRNA markers of neoplastic tumors; these included c-kit in mast cell tumor, microphthalmia-associated transcription factor in malignant melanoma, ionized calcium-binding adapter molecule-1 in histiocytic sarcoma,
and alkaline phosphatase in osteosarcoma. A strong staining signal was obtained by these 4 targets in each canine malignancy. These results support the use of RNAscope ISH for definitive diagnosis in canine malignancies. |
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Bibliography: | 0830-9000(20201001)84:4L.319;1- |
ISSN: | 0830-9000 1928-9022 |