Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

• Published in: Molecular nutrition & food research Vol. 57; no. 6; pp. 1100 - 1109
• Main Authors: Santos, Keity Souza, Gadermaier, Gabriele, Vejvar, Eva, Arcuri, Helen Andrade, Galvão, Clovis Eduardo, Yang, Ariana Campos, Resende, Virgínia Maria Ferreira, de Oliveira Martins, Carlo, Himly, Martin, Mari, Adriano, Liso, Marina, Pomponi, Debora, Breiteneder, Heimo, Wagner, Stefan, Kalil, Jorge, Ferreira, Fátima, Castro, Fábio Fernandes Morato
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.06.2013; Wiley
• Subjects: Adult; Allergens - genetics; Allergens - immunology; Allergic diseases; Amino Acid Sequence; Antigens, Plant - genetics; Antigens, Plant - immunology; Biological and medical sciences; Brazil; Circular Dichroism; Cloning, Molecular; Cross Reactions - immunology; Digestive allergic diseases; Feeding. Feeding behavior; Female; Food allergy; Food Hypersensitivity - blood; Food Hypersensitivity - diagnosis; Food Hypersensitivity - immunology; Fundamental and applied biological sciences. Psychology; Hev b 5; Humans; IgE cross-reactivity; Immune Sera; Immunoglobulin E - immunology; Immunopathology; Intrinsically disordered protein; Latex - immunology; Latex Hypersensitivity - blood; Latex Hypersensitivity - immunology; Latex-fruit syndrome; Male; Manihot - chemistry; Manihot - immunology; Medical sciences; Middle Aged; Models, Molecular; Molecular Sequence Data; New allergen; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - immunology; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Brazil; Food allergy; Toxicity; IgE; Male; IgE cross-reactivity; Dicotyledones; Angiospermae; Cross reaction; Euphorbiaceae; Food; Human; Immunopathology; Latex-fruit syndrome; Fruit; Nutrition; Manihot esculenta; New allergen; Latex; Protein; Syndrome; Plant origin; Hev b 5; Digestive diseases; Spermatophyta; Intrinsically disordered protein; Allergen

Scope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods an...