Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex

• Published in: Molecular nutrition & food research Vol. 57; no. 6; pp. 1100 - 1109
• Main Authors: Santos, Keity Souza, Gadermaier, Gabriele, Vejvar, Eva, Arcuri, Helen Andrade, Galvão, Clovis Eduardo, Yang, Ariana Campos, Resende, Virgínia Maria Ferreira, de Oliveira Martins, Carlo, Himly, Martin, Mari, Adriano, Liso, Marina, Pomponi, Debora, Breiteneder, Heimo, Wagner, Stefan, Kalil, Jorge, Ferreira, Fátima, Castro, Fábio Fernandes Morato
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.06.2013; Wiley
• Subjects: Adult; Allergens - genetics; Allergens - immunology; Allergic diseases; Amino Acid Sequence; Antigens, Plant - genetics; Antigens, Plant - immunology; Biological and medical sciences; Brazil; Circular Dichroism; Cloning, Molecular; Cross Reactions - immunology; Digestive allergic diseases; Feeding. Feeding behavior; Female; Food allergy; Food Hypersensitivity - blood; Food Hypersensitivity - diagnosis; Food Hypersensitivity - immunology; Fundamental and applied biological sciences. Psychology; Hev b 5; Humans; IgE cross-reactivity; Immune Sera; Immunoglobulin E - immunology; Immunopathology; Intrinsically disordered protein; Latex - immunology; Latex Hypersensitivity - blood; Latex Hypersensitivity - immunology; Latex-fruit syndrome; Male; Manihot - chemistry; Manihot - immunology; Medical sciences; Middle Aged; Models, Molecular; Molecular Sequence Data; New allergen; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - immunology; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Brazil; Food allergy; Toxicity; IgE; Male; IgE cross-reactivity; Dicotyledones; Angiospermae; Cross reaction; Euphorbiaceae; Food; Human; Immunopathology; Latex-fruit syndrome; Fruit; Nutrition; Manihot esculenta; New allergen; Latex; Protein; Syndrome; Plant origin; Hev b 5; Digestive diseases; Spermatophyta; Intrinsically disordered protein; Allergen
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201200433

Scope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods and results The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross‐inhibition assays suggest a possible involvement of this allergen in the latex‐fruit syndrome. Conclusion Man e5, the first purified allergen from manioc demonstrates IgE cross‐reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.