Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

• Published in: International journal of immunogenetics Vol. 41; no. 1; pp. 74 - 80
• Main Authors: Sugumar, Thennarasu, Pugalenthi, Ganesan, Harishankar, Murugesan, Dhinakar Raj, G
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.02.2014
• Subjects: Animals; Bubalus bubalis; Buffaloes - genetics; Cloning, Molecular; Granulocyte-Macrophage Colony-Stimulating Factor - chemistry; Granulocyte-Macrophage Colony-Stimulating Factor - genetics; Sequence Analysis, DNA
• ISSN: 1744-3121; 1744-313X
• DOI: 10.1111/iji.12074

Summary Granulocyte–macrophage colony‐stimulating factor (GM‐CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM‐CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM‐CSF. Buffalo GM‐CSF has been compared with 17 mammalian GM‐CSFs using multiple sequence alignment and phylogenetic tree. Three‐dimensional model for buffalo GM‐CSF and human receptor complex was built using homology modelling to study cross‐reactivity between two species. Detailed analysis was performed to study GM‐CSF interface and various interactions at the interface.