Synthesis and characterization of Bi2O3/HSA core-shell nanoparticles for X-ray imaging applications

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 101B; no. 1; pp. 131 - 138
• Main Authors: Aviv, Hagit, Bartling, Soenke, Grinberg, Igor, Margel, Shlomo
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2013; Wiley-Blackwell
• Subjects: Animals; Biological and medical sciences; Bismuth - chemistry; bismuth oxide nanoparticles; Contrast media. Radiopharmaceuticals; CT contrast agents; HSA coating; Humans; Male; Medical sciences; Microscopy, Electron, Transmission; Nanoparticles; Pharmacology. Drug treatments; radiopacity; Rats; Serum Albumin - chemistry; Spectrophotometry, Ultraviolet; thermal decomposition; X-Rays; Human; Coating material; Radiodiagnosis; thermal decomposition; Bismuth oxide; bismuth oxide nanoparticles; Serum albumin; CT contrast agents; radiopacity; Protein; Synthesis; Preparation; Medical imagery; Biomaterial; Computerized axial tomography; Contrast media; HSA coating; Biomedical engineering
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.32826

Bismuth oxide nanoparticles of 12.1 ± 3.0 nm diameter were prepared by thermal decomposition of bismuth acetate dissolved in ethylene glycol in the presence of an oxidizing agent. Functionalization and stabilization of the hydrophobic Bi2O3 nanoparticles was accomplished by coating these core nanoparticles with human serum albumin (HSA), via a precipitation process. The formed Bi2O3/HSA core‐shell nanoparticles were of 15.2 ± 3.5 nm diameter. Elemental analysis measurements indicated that the bismuth weight % of the Bi2O3/HSA core‐shell nanoparticles is 72.9. The crystalline structure of these nanoparticles was examined by XRD. The radiopacity of these nanoparticles was demonstrated in vitro and in vivo by a CT scanner. In ovo and in vivo trials proved the safety of these Bi2O3/HSA core‐shell nanoparticles. In the future, we plan to extend this study particularly for molecular imaging applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 131–138, 2013.