Direct in Situ Observation of a Lipid Monolayer−DNA Complex at the Air−Water Interface by X-ray Reflectometry

• Published in: Langmuir Vol. 15; no. 16; pp. 5193 - 5196
• Main Authors: KAGO, Keitaro, MATSUOKA, Hideki, YOSHITOME, Ryuji, YAMAOKA, Hitoshi, IJIRO, Kuniharu, SHIMOMURA, Masatsugu
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 03.08.1999
• Subjects: Biological and medical sciences; DNA; Fundamental and applied biological sciences. Psychology; Interfaces (materials); Lipids; Molecular biophysics; Molecular dynamics; Molecular structure; Structure (composition); Surface properties. Adsorption; Surface roughness; X ray analysis; Cationic complex; Monolayer; Molecular structure; DNA; Surface properties; Reflectometry; Lipids; Molecular complex; Air water interface; Physical properties
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la981352a

Direct in situ observation of the fine structure of the cationic lipid monolayer - DNA complex on a water surface has been carried out by using an air - water interface X-ray reflectivity (XR) instrument. Interestingly, the thickness of the DNA layer was markedly thinner than the geometry of the cylindrical DNA molecule when the complex was deposited on the solid substrates; the thickness was determined to be 11 angstrom by XR measurement in a dried state, while the diameter of the DNA molecule is about 24 angstrom. However, the thickness in the complex on a water surface was estimated by in situ XR measurement to be about 25-28 angstrom, which is comparable to the geometry of the DNA molecule. Thus, the anomalously thin thickness was due to some experimental treatments, such as deposition on a solid substrate and/or drying. The structure of the monolayer and monolayer - polymer complex on the solid substrates in a dried state is not the same as that on a water surface. The possibility of some dynamic fluctuation of its structure was also suggested. These results strongly indicate the importance of a direct in situ study such as by the XR technique for the structural study of the monolayer and monolayer complex on a water surface.