Protective Effects of Aucubin on H2O2-Induced Apoptosis in PC12 Cells

• Published in: Phytotherapy research Vol. 26; no. 3; pp. 369 - 374
• Main Authors: Xue, Hong-Yu, Gao, Gui-Zhen, Lin, Qiu-Ye, Jin, Li-Ji, Xu, Yong-Ping
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.03.2012; Wiley
• Subjects: Animals; Antioxidants - metabolism; Apoptosis; aucubin; Biological and medical sciences; Catalase - metabolism; Cell Nucleus Shape; Cell Survival; Enzyme Activation; Flow Cytometry; General pharmacology; Glutathione Peroxidase - metabolism; hydrogen peroxide; Hydrogen Peroxide - adverse effects; Hydrogen Peroxide - metabolism; Iridoid Glucosides - pharmacology; L-Lactate Dehydrogenase - metabolism; Malondialdehyde - metabolism; Medical sciences; Neuroprotective Agents - pharmacology; oxidant-antioxidant balance; Oxidative Stress; PC12 Cells; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Rats; Staining and Labeling; Superoxide Dismutase - metabolism; Iridoid; Terpenoid; Hydrogen peroxide; Pharmacognosy; oxidant―antioxidant balance; Antioxidant; aucubin; In vitro; PC12 cells; Medicinal plant; Prevention; Cell death; Plant origin; Monoterpene; Glycoside; Apoptosis
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.3562

The present study investigated the neuroprotective effects of aucubin on hydrogen peroxide (H2O2)‐induced apoptosis in PC12 cells. Exposure of PC12 cells to 0.25 mm H2O2 induced a leakage of lactate dehydrogenase and decreased cell viability, as shown by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide assay. In a dose over 0.1 mm, aucubin increased PC12 cellular viability and markedly attenuated H2O2‐induced apoptotic cell death. Quantitation of apoptosis by flow cytometry indicated that aucubin inhibited H2O2‐induced apoptosis in PC12 cells. Nuclear damage was alleviated by aucubin, as shown by Hoechst staining. In addition, the levels of malondialdehyde were reduced and the activity of superoxide dismutase, catalase and glutathione peroxidase was augmented in these cells. These results indicated that aucubin inhibited H2O2‐induced apoptosis in PC12 cells through regulation of the endogenous oxidant–antioxidant balance. Our results suggest that aucubin is a potential protective agent for the treatment of oxidative‐stress‐induced neurodegenerative disease. Copyright © 2011 John Wiley & Sons, Ltd.