Determination of sphinganine, sphingosine and Sa/So ratio in urine of humans exposed to dietary fumonisin B1

• Published in: Food additives and contaminants Vol. 18; no. 3; pp. 263 - 269
• Main Authors: Qiu, Maofeng, Liu, Xiumei
• Format: Journal Article Conference Proceeding
• Language: English
• Published: London Taylor & Francis Group 01.03.2001; Taylor & Francis
• Subjects: Adult; Biological and medical sciences; Biomarkers - urine; Carboxylic Acids - administration & dosage; Carcinogens, Environmental - administration & dosage; Cereal and baking product industries; Chromatography, High Pressure Liquid - methods; Environmental Monitoring - methods; Enzyme Inhibitors - urine; Female; Food Contamination; Food industries; Food toxicology; Fumonisins; Fumonisins Sphingosine Sphinganine Human Urine; Fundamental and applied biological sciences. Psychology; Humans; Male; Mycotoxins - administration & dosage; Protein Kinase C - antagonists & inhibitors; Sphingosine - analogs & derivatives; Sphingosine - urine; Zea mays - chemistry; Human; Urine; Mycotoxin; Sphingolipid; Biological marker; Corn; Biosynthesis; Liquid chromatography; Feeding; Toxin; Cereal; Fumonisin; Mechanism of action; Biological contamination; Sphingosin; Quantitative analysis
• ISSN: 0265-203X; 1464-5122
• DOI: 10.1080/02652030117470

Fumonisin B 1 (FB 1 ) is an inhibitor of sphinganine N-acyltransferase and the increase in the sphinganine/sphingosine (Sa/So) ratio in urine or serum has been proposed as a biomarker to evaluate exposure to fumonisins. The objectives of this study were to (1) develop a liquid chromatographic method sufficiently sensitive to determine the low concentration of free Sa in male human urine, and (2) analyse So and Sa in human urine and monitor the Sa/So ratio in urine of humans exposed to FB 1 in corn diets over 1 month. The liquid chromatographic method involved isolation from human urine of exfoliated cells followed by an extraction of free sphingoid bases and their separation and quantification by high performance liquid chromatography. The detection limits for So and Sa were 0.15ng/ml in female urine (2ml used) and 0.005ng/ml in male urine (60ml used). Twenty-eight healthy adult volunteers consumed for 1 month a normal diet containing their homegrown corn potentially contaminated with FB 1 . Immediately preceding the start of the test, morning urine samples for the determination of So and Sa were collected from each person, and the corn samples used in cooking were obtained from each family for the determination of FB 1 . At the end of the test period, morning-urine samples were collected from each person and analysed again. The daily FB 1 intakes were estimated and used to assess the relationship between them and the urinary Sa/So ratios in humans exposed to dietary FB 1 over 1 month. All the home grown corn samples contained FB 1 ranging from 0.08 to 41.1mg/kg, and the estimated daily FB 1