Parallel increase in community use of fosfomycin and resistance to fosfomycin in extended-spectrum β-lactamase (ESBL)-producing Escherichia coli

• Published in: Journal of antimicrobial chemotherapy Vol. 65; no. 11; pp. 2459 - 2463
• Main Authors: Oteo, Jesús, Bautista, Verónica, Lara, Noelia, Cuevas, Oscar, Arroyo, Margarita, Fernández, Sara, Lázaro, Edurne, de Abajo, Francisco J., Campos, José
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.2010
• Subjects: Aged; Aged, 80 and over; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial Typing Techniques; beta-Lactamases - biosynthesis; Biological and medical sciences; CTX-M-15; DNA Fingerprinting; Drug Resistance, Bacterial; Drug Utilization - statistics & numerical data; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - isolation & purification; Escherichia coli Infections - classification; Escherichia coli Infections - microbiology; Female; Fosfomycin - pharmacology; Fosfomycin - therapeutic use; Genotype; Humans; Male; Medical sciences; Microbial Sensitivity Tests; Pharmacology. Drug treatments; Phylogeny; phylogroup B2; Polymerase Chain Reaction; Sequence Analysis, DNA; serotype O25b; Serotyping; Spain; Urinary Tract Infections - microbiology; Spain; Typing; Escherichia coli; Extended-spectrum β-lactamase; CTX-M-15; serotype O25b; Fosfomycin; Resistance; Antibiotic; Bacteria; Phosphonic acid derivatives; Serotype; Antibacterial agent; phylogroup B2; Enterobacteriaceae
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkq346

Objectives To document fosfomycin susceptibility of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC), analyse trends in fosfomycin use and investigate fosfomycin resistance in ESBL-EC isolated from urinary tract infections (UTIs). Methods Twenty-seven Spanish hospitals participating in the European Antimicrobial Resistance Surveillance Network were requested to collect up to 10 sequential ESBL-EC for centralized susceptibility testing and typing. EUCAST guidelines were followed for antibiotic susceptibility testing, and blaESBL type, phylogroups and O25b serotype were determined by PCR and sequencing. In addition, the trend in fosfomycin resistance among ESBL-EC causing UTIs was determined in 9 of the 27 hospitals. Total fosfomycin use for ambulatory care was established by WHO-recommended methods. Results A total of 231 ESBL-EC (42.4% CTX-M-15, 34.2% SHV-12 and 23.4% CTX-M-14) were collected. The overall rate of fosfomycin resistance was 9.1%, but varied according to ESBL type (5.6% of CTX-M-14 isolates, 5.1% of SHV-12 and 15.3% of CTX-M-15). Of 67 O25b/B2 isolates, 11 (16.4%) were fosfomycin resistant. Predictors of infection with fosfomycin-resistant ESBL-EC were O25b/phylogroup B2 isolates, female gender and nursing home residence. Among 114 197 UTIs caused by E. coli 4740 (4.2%) were due to ESBL-EC. Fosfomycin resistance increased in these isolates from 4.4% (2005) to 11.4% (2009). The use of fosfomycin grew from 0.05 defined daily doses per 1000 inhabitants per day (1997) to 0.22 (2008), a 340% increase. Conclusions Key factors related to increased fosfomycin resistance in ESBL-EC causing UTIs could be the rapid growth in community use of fosfomycin, the widespread distribution of the 025b/B2 E. coli clone and the existence of a susceptible population comprising women residing in nursing home facilities.