Identification of α2 Macroglobulin as a Major Serum Ghrelin Esterase

Fishing for a protein: An analogue of the appetite‐stimulating hormone ghrelin containing a phosphonofluoridate moiety and a terminal alkyne functions as a probe to capture the protein α2 macroglobulin (red; see picture). The extraction of the protein is highly selective and a previously undocumente...

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Published inAngewandte Chemie International Edition Vol. 50; no. 45; pp. 10699 - 10702
Main Authors Eubanks, Lisa M., Stowe, G. Neil, De Lamo Marin, Sandra, Mayorov, Alexander V., Hixon, Mark S., Janda, Kim D.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 04.11.2011
WILEY‐VCH Verlag
Subjects
alkynes
alpha-Macroglobulins - metabolism
Amino Acid Sequence
Animals
azides
click chemistry
enzymes
Esterases - blood
Ghrelin - blood
Ghrelin - metabolism
Humans
Molecular Sequence Data
peptides
Rats
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Summary:Fishing for a protein: An analogue of the appetite‐stimulating hormone ghrelin containing a phosphonofluoridate moiety and a terminal alkyne functions as a probe to capture the protein α2 macroglobulin (red; see picture). The extraction of the protein is highly selective and a previously undocumented catalytic activity of α2 macroglobulin as a hydrolase for ghrelin has been identified.
Bibliography:ArticleID:ANIE201104512
National Institute of Health - No. R01-DK092191
istex:7C35D5F249394DEF3DD4677FFA89AF0A562A8B63
The Skaggs Institute for Chemical Biology
ark:/67375/WNG-JHHGW3D3-4
This work was supported by The Skaggs Institute for Chemical Biology and the National Institute of Health under grant number R01-DK092191.
This work was supported by The Skaggs Institute for Chemical Biology and the National Institute of Health under grant number R01‐DK092191.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201104512