Cytotoxicity of synthesized Iron Oxide nanoparticles: Toward novel biomarkers of colon cancer

• Published in: 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society Vol. 2014; pp. 6179 - 6182
• Main Authors: Raji, M. Ahmadzadeh, Amara, M., Amoabediny, G., Tajik, P., Barin, A., Magierowski, S., Ghafar-Zadeh, E.
• Format: Conference Proceeding Journal Article
• Language: English
• Published: United States IEEE 01.01.2014
• Subjects: Aptamers, Nucleotide - chemistry; Biomarkers - analysis; Cancer; Colon; Colonic Neoplasms - metabolism; Colonic Neoplasms - pathology; Ferrosoferric Oxide - chemistry; HCT116 Cells; Humans; Iron; Magnetic field measurement; Magnetic resonance imaging; Magnetic separation; Magnetite Nanoparticles - chemistry; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microscopy, Electron, Transmission; Nanoparticles; Particle Size
• ISSN: 1094-687X; 1557-170X; 1558-4615
• DOI: 10.1109/EMBC.2014.6945040

In this paper we present the preliminary results of a novel biological analysis platform for early colon cancer detection using magnetic separation of magnetized markers. The platform consists of a microfluidic structure integrated with biosensors. Super-Paramagnetic Iron Oxide nanoparticles (SPIO-NPs) were functionalized with purified DNA Aptamer and their synthesis is described. In this paper, we also present the physicochemical results of the synthesized SPIO/Au-NPs characterized by TEM and XRD. Toxicity of our synthesized biomarkers on HCT116 cell line is discussed. Based on our findings, a concentration of 1mg/ml of our biomarkers added to 5 × 105 cells per well has no effect the viability of the human cells even after 24 hours.