Investigation of RNA-Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes

Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by...

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Published inAngewandte Chemie (International ed.) Vol. 51; no. 38; pp. 9530 - 9534
Main Authors Lombès, Thomas, Moumné, Roba, Larue, Valéry, Prost, Elise, Catala, Marjorie, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent, Tisné, Carine
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 17.09.2012
WILEY‐VCH Verlag
Wiley
Subjects
19F NMR spectroscopy
aminoglycosides
Aminoglycosides - chemistry
Chemistry
Chemistry, Multidisciplinary
Fluorescent Dyes - chemistry
Fluorine - chemistry
Framycetin - chemistry
Ligands
Magnetic Resonance Spectroscopy
Neomycin - chemistry
Paromomycin - chemistry
Physical Sciences
reporter ligands
riboswitches
RNA
RNA - chemistry
Science & Technology
SITE
RNA
SMALL MOLECULES
SPECIFICITY
RECOGNITION
DRUG DISCOVERY
19F NMR spectroscopy
RIBOSOMAL-RNA
TARGETING RNA
DISEASE
aminoglycosides
riboswitches
reporter ligands
BINDING
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Summary:Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by its ligand.
Bibliography:ANRS
ArticleID:ANIE201204083
CNRS
ANR
ark:/67375/WNG-8T1NV333-D
istex:388AA748CEE3D5FFFF1353480F9C7E3B34DBB25B
Financial support from CNRS Bettencourt Schueller Foundation (PhD grant to T.L.), ANRS (grant to R.M.) and ANR (research project PCV TriggeRNA) is acknowledged.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201204083