Induction of GD3 Ganglioside by Adenovirus E1A Gene 13S- and 12S-mRNA Products in Rat 3Y1 Cells

• Published in: Journal of biochemistry (Tokyo) Vol. 107; no. 5; pp. 740 - 742
• Main Authors: Sanai, Yutaka, Oda, Kinichiro, Nagai, Yoshitaka
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 1990
• Subjects: Adenovirus Early Proteins; Animals; Biological and medical sciences; Cell Line; Cell physiology; Dexamethasone - pharmacology; DNA, Recombinant - genetics; Fibroblasts - drug effects; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Gangliosides - biosynthesis; Gangliosides - genetics; Gene Expression; Molecular and cellular biology; Oncogene Proteins, Viral - genetics; Rats; Responses to growth factors, tumor promotors, other factors; RNA, Messenger - biosynthesis; Adenoviridae; Rat; HPLC chromatography; Lipids; Recombinant DNA; Cell transformation; Immunological method; Qualitative analysis; Type B oncovirus; Mouse mammary tumor virus; Oncovirinae; Adenovirus 12; Heterologous system; Induction; Rodentia; Retroviridae; Virus; Thin layer chromatography; Vertebrata; Mammalia; Cell line; Complementary DNA; Ganglioside; Mastadenovirus; Kinetics
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a123118

The effect of the expression of the human adenovirus type 12 E1A gene on ganglioside composition of rat 3Y1 cells was investigated using cell lines established by introduction of recombinant vector DNA containing the E1A 13S- or 12S-mRNA cDNA placed downstream of the hormone-inducible promoter of mouse mammary tumor virus. Ganglioside GD3 was newly detected after switching on of either 13S- or 12S-mRNA cDNA by addition of dexamethasone. A kinetic study indicated that GD3 was expressed 8 h after additon of dexamethasone. Gangloside GM2 was also accumulated by induction of 13S-mRNA of adenovirus E1A gene. The results indicated that 13S- or 12S-mRNA product alone has the ability to induce GD3 ganglioside in rat 3Y1 cells.