Genetic Manipulation of Rubisco: Chromatium vinosum rbcL is expressed in Nicotiana tabacum but does not form a functional protein

Summary N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits w...

Full description

Saved in:
Bibliographic Details
Published inAnnals of applied biology Vol. 140; no. 1; pp. 13 - 19
Main Authors MADGWICK, P J, COLLIVER, S P, BANKS, F M, HABASH, D Z, DULIEU, H, PARRY, M A J, PAUL, M J
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.02.2002
Blackwell
Subjects
Biological and medical sciences
C. vinosum
Fundamental and applied biological sciences. Psychology
photosynthesis
rbcL
Rubisco
transgenic
Online AccessGet full text

Cover

More Information
Summary:Summary N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.
Bibliography:ArticleID:AAB13
istex:2DD32666CE0FA7A2CF1D9A24E045AB8455F22B30
ark:/67375/WNG-6DB040ZJ-R
ISSN:0003-4746
1744-7348
DOI:10.1111/j.1744-7348.2002.tb00152.x