Three New Phenolic Compounds from a Manipulated Plant Cell Culture, Mirabilis jalapa

• Published in: Journal of natural products (Washington, D.C.) Vol. 64; no. 3; pp. 313 - 317
• Main Authors: YANG, Shu-Wei, UBILLAS, Rosa, MCALPINE, James, STAFFORD, Angela, ECKER, Dawn M., TALBOT, M. Kelly, ROGERS, Bruce
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.03.2001; Amer Chemical Soc; American Society of Pharmacognosy
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Biological and medical sciences; Biotechnology; Candida albicans - drug effects; Cells, Cultured; Chemistry, Medicinal; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; General pharmacology; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Magnoliopsida - chemistry; Medical sciences; Methods. Procedures. Technologies; Microbial Sensitivity Tests; Miscellaneous; Pharmacognosy. Homeopathy. Health food; Pharmacology & Pharmacy; Pharmacology. Drug treatments; Phenols - chemistry; Phenols - isolation & purification; Plant cells and fungal cells; Plant Sciences; Science & Technology; ROOTS; BOERHAAVIA-DIFFUSA L; CONSTITUENTS; Candida albicans; Cell culture; Pharmacognosy; Tetracyclic compound; Nyctaginaceae; Oxygen heterocycle; Flavonoid; In vitro; Biological activity; Medicinal plant; Fungi; Antifungal agent; Polyphenol; Dicotyledones; Angiospermae; Plant origin; Bicyclic compound; Isolation; Spermatophyta; Aromatic compound; Fungi Imperfecti; Genetically modified organism; Thallophyta; Structural analysis
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np0004092

Bioassay-guided fractionation of an organic extract of the cell mass from a manipulated plant cell culture of Mirabilis jalapa led to the isolation and subsequent identification of three new phenolic compounds, 1, 2, and 3. The isoflavone 1 and dehydrorotenoid 2 were identified as the principal antifungal principles from this plant cell culture with IC50's of 25 and 48 mug/mL, respectively, against the test organism, Candida albicans DSY1024. The rotenoid 3 was inactive at 200 mug/mL in this assay.